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This article will show you how to measure a daphnia's heartbeat. This a simple process and is easily done by all ages and of all levels of knowledge.
What will I need? Edit
- A few daphnia
- Microscope and slides
- Paper and pen
- Second person
- Set up you microscope ready.
- Then, using your pipette, suck up a daphnia and put it on a slide. You may wish to remove some water so it is more clear under the microscope but leave enough for the daphnia to survive.
- Put the daphnia under the microscope and watch for it's heart beat. You can normally see this on what seems to be its back. It looks like a small rise and fall. If you cannot see anything or the daphnia will not come in your view for a while you may need to remove some water. If this does not work try it with another daphnia
- Once you find the heartbeating get your second person to start the stopwatch. Tell them to stop it at 20 seconds
- Then with your pen tap hard dots going in an 'S' shape on paper for every time you see the heart beat.
- After, on your calculator, times the amount of dots you marked by 3 to get your beats per minutes (BPM).